Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis.<\/p>\n
To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing\u00a0Klebsiella pneumoniae<\/i>\u00a0sepsis<\/p>\nMETHODS:<\/h2>\n
Several ESBL inoculums ranging from 1.5x109<\/sup><\/span>\u00a0colony-forming units per milliliter (CFU\/mL) to 2.0x1010<\/sup><\/span>CFU\/mL were administered by peritoneal injection in adults Wistar rats. Outcomes and microbiological data of quantitative peritoneal and blood cultures were observed in untreated animals. Animals which received 2.0x1010<\/sup><\/span>CFU\/mL inoculums were treated with single meropenem dose (30mg\/kg) after one hour and those which received 1.0x1010<\/sup><\/span>CFU\/mL inoculums were treated immediately with three doses of meropenem 50 mg\/kg. Outcomes were observed for 24 hours after inoculation.<\/p>\n Solutions with 1.5 x109<\/sup><\/span>\u00a0and 6.0x109<\/sup><\/span>\u00a0CFU\/mL were not lethal within 24 hours. Inoculums of 1.0x1010<\/sup><\/span>CFU\/mL were lethal in 80% and solutions with 2.0x1010<\/sup><\/span>\u00a0CFU\/mL were lethal in 100% of animals. ESBL lethal sepsis (1.0x1010<\/sup><\/span>CFU\/mL) was treated immediately with 50 mg\/kg of meropenem every eight hours for 24 hours and presented 40% mortality compared with 80% mortality of the control group (p=0.033). Quantitative cultures of peritoneal fluid presented 104<\/sup><\/span>CFU\/mL or less for treated animals compared to more than 105<\/sup><\/span>\u00a0for untreated animals (p=0.001).<\/p>\n Inoculums of 1.0x1010<\/sup><\/span>CFU\/mL achieved the best results to study a model of lethal sepsis and this model of treatment of carbapenem-susceptible\u00a0Enterobacteriaceae\u00a0<\/i>can serve as control to further evaluation of treatment of carbapenemase-producing\u00a0Enterobacteriaceae\u00a0<\/i>models.<\/p>","protected":false},"excerpt":{"rendered":" BACKGROUND: Animal models are useful to evaluate the efficacy of antimicrobials in experimental sepsis. AIM: To elucidate the steps of producing an experimental model for the treatment of extended-spectrum beta-lactamase (ESBL)-producing\u00a0Klebsiella pneumoniae\u00a0sepsis METHODS: Several ESBL inoculums ranging from 1.5x109\u00a0colony-forming units per milliliter (CFU\/mL) to 2.0x1010CFU\/mL were administered by peritoneal injection in adults Wistar rats. Outcomes […]<\/p>","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_lmt_disableupdate":"no","_lmt_disable":"","footnotes":""},"categories":[9],"tags":[700,1292,698,539],"acf":[],"modified_by":null,"_links":{"self":[{"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/posts\/5013"}],"collection":[{"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/comments?post=5013"}],"version-history":[{"count":2,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/posts\/5013\/revisions"}],"predecessor-version":[{"id":5017,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/posts\/5013\/revisions\/5017"}],"wp:attachment":[{"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/media?parent=5013"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/categories?post=5013"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/tags?post=5013"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}RESULTS:<\/h2>\n
CONCLUSION:<\/h2>\n