{"id":5925,"date":"2024-12-18T15:40:27","date_gmt":"2024-12-18T18:40:27","guid":{"rendered":"https:\/\/revistaabcd.org.br\/?p=5925"},"modified":"2024-12-18T15:40:27","modified_gmt":"2024-12-18T18:40:27","slug":"use-of-decellularized-human-amniotic-membrane-in-intestinal-anastomoses-a-study-in-rats-treated-with-5-fluorouracil","status":"publish","type":"post","link":"https:\/\/revistaabcd.org.br\/pt-br\/use-of-decellularized-human-amniotic-membrane-in-intestinal-anastomoses-a-study-in-rats-treated-with-5-fluorouracil\/","title":{"rendered":"USE OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN INTESTINAL ANASTOMOSES: A STUDY IN RATS TREATED WITH 5-FLUOROURACIL"},"content":{"rendered":"<h2>BACKGROUND<strong>:<\/strong><\/h2>\n<p>Nowdays, more relevant applications of perinatal derivatives, such as amniotic membrane (AM), are emerging in our environment as a source of biomaterials for use in different healing processes. The study of anastomosis healing associated with antimetabolic drugs such as 5-fluorouracil (5-FU) is a potential target of AM.<\/p>\n<h2>AIMS:<\/h2>\n<p>To evaluate the healing effects of AM in rats treated with 5-FU at a dose of 20 mg\/kg on the seventh day of postoperative evolution, regarding the parameters percentage of type I collagen (mature), cell viability, microvascular density and formation of granulation tissue.<\/p>\n<h2>METHODS:<\/h2>\n<p>Thirty-two Wistar rats were used, submitted to colotomy and colorraphy, separated into four groups of eight, which received different treatments daily, intraperitoneally, until the day of sacrifice: saline solution (C), 20 mg\/kg 5-FU, 20 mg\/kg 5-FU and AM.<\/p>\n<h2>RESULTS:<\/h2>\n<p>Treatment with 20 mg\/kg of 5-FU, on the seventh postoperative day, induced adverse effects on the anastomotic healing process, evidenced by a decrease in the percentage of type I (mature) collagen, cell viability, microvascular density, fibrin-leukocyte scab formation and angiofibroblast proliferation; the use of AM under these conditions induced an improvement in the percentage of type I (mature) collagen.<\/p>\n<h2>CONCLUSIONS:<\/h2>\n<p>Treatment with 20 mg\/kg of 5-FU on the seventh postoperative day induced adverse effects on the anastomotic healing process, and the use of AM under these conditions induced an improvement in the percentage of type I (mature) collagen.<\/p>","protected":false},"excerpt":{"rendered":"<p>BACKGROUND: Nowdays, more relevant applications of perinatal derivatives, such as amniotic membrane (AM), are emerging in our environment as a source of biomaterials for use in different healing processes. The study of anastomosis healing associated with antimetabolic drugs such as 5-fluorouracil (5-FU) is a potential target of AM. AIMS: To evaluate the healing effects of [&hellip;]<\/p>","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_lmt_disableupdate":"","_lmt_disable":"","footnotes":""},"categories":[9],"tags":[1417,1277,914,1418,1301],"class_list":["post-5925","post","type-post","status-publish","format-standard","hentry","category-original-article","tag-amnion","tag-combination","tag-drug-therapy","tag-membranes","tag-surgical-anastomosis"],"acf":[],"modified_by":"Giovani","_links":{"self":[{"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/posts\/5925"}],"collection":[{"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/comments?post=5925"}],"version-history":[{"count":2,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/posts\/5925\/revisions"}],"predecessor-version":[{"id":5928,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/posts\/5925\/revisions\/5928"}],"wp:attachment":[{"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/media?parent=5925"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/categories?post=5925"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/revistaabcd.org.br\/pt-br\/wp-json\/wp\/v2\/tags?post=5925"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}