30 Mar, 2020

Conservation of both hematocrit and liver regeneration in hepatectomies: a vascular occlusion approach in rats

Eduardo Augustus MALINOWSKI
Jorge Eduardo Fouto MATIAS
Ana Paula PERCICOTE
Thaísa NAKADOMARI
Rogério ROBES
Ricardo Rasmussen PETTERLE
Lúcia De NORONHA
Jose Luiz De GODOY
DOI: 10.1590/0102-672020190001e1
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Background:

Hepatectomies promote considerable amount of blood loss and the need to administrate blood products, which are directly linked to higher morbimortality rates. The blood-conserving hepatectomy (BCH) is a modification of the selective vascular occlusion technique. It could be a surgical maneuver in order to avoid or to reduce the blood products utilization in the perioperative period.

Aim:

To evaluate in rats the BCH effects on the hematocrit (HT) variation, hemoglobin serum concentration (HB), and on liver regeneration.

Methods:

Twelve Wistar rats were divided into two groups: control (n=6) and intervention (n=6). The ones in the control group had their livers partially removed according to the Higgins and Anderson technique, while the rats in the treatment group were submitted to BCH technique. HT and HB levels were measured at day D0, D1 and D7. The rate between the liver and rat weights was calculated in D0 and D7. Liver regeneration was quantitatively and qualitatively evaluated.

Results:

The HT and HB levels were lower in the control group as of D1 onwards, reaching an 18% gap at D7 (p=0.01 and p=0.008, respectively); BCH resulted in the preservation of HT and HB levels to the intervention group rats. BCH did not alter liver regeneration in rats.

Conclusion:

The BCH led to beneficial effects over the postoperative HT and serum HB levels with no setbacks to liver regeneration. These data are the necessary proof of evidence for translational research into the surgical practice.


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