Revista ABCd (São Paulo). 17 Jun, 2022

IMMUNOHISTOCHEMICAL DETECTION OF L CELLS IN GASTROINTESTINAL TRACT MUCOSA OF PATIENTS AFTER SURGICAL TREATMENT FOR CONTROL OF TYPE 2 DIABETES MELLITUS

Priscila Costa ESTABILE
Mara Cristina de ALMEIDA
Eduardo Bauml CAMPAGNOLI
Marco Aurelio SANTO
Marcos Ricardo da Silva RODRIGUES
Fábio Quirillo MILLÉO
Roberto Ferreira ARTONI
DOI: 10.1590/0102-672020210002e1651

ABSTRACT - BACKGROUND:

Type 2 diabetes mellitus (T2DM) is a disease of global impact that has led to an increase in comorbidities and mortality in several countries. Immunoexpression of the incretin hormones such as glucagon-like peptide-1 (GLP-1) and peptide YY (3-36) (PYY3-36) can be used as a scorer in the gastrointestinal tract to analyze L-cell activity in response to T2DM treatment.

OBJECTIVE:

This study aimed to investigate the presence, location, and secretion of L cells in the small intestine of patients undergoing the form of bariatric surgery denominated adaptive gastroenteromentectomy with partial bipartition.

METHODS:

Immunohistochemical assays, quantitative real-time polymerase chain reaction (qPCR), and Western blot analysis were performed on samples of intestinal mucosa from patients with T2DM in both the preoperative and postoperative periods.

RESULTS:

All results were consistent and indicated basal expression and secretion of GLP-1 and PYY3-36 incretins by L cells. A greater density of cells was demonstrated in the most distal portions of the small intestine. No significant difference was found between GLP-1 and PYY3-36 expression levels in the preoperative and postoperative periods because of prolonged fasting during which the samples were collected.

CONCLUSION:

The greater number of L cells in activity implies better peptide signaling, response, and functioning of the neuroendocrine system.


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